Define Counting Frame
This command allows you to define the XY size of the counting frame used with the Fractionator, Optical Fractionator and Linear Optical Disector probes. The counting frame disappears immediately after it has been defined. It is only displayed during the execution of the probes that use it.
If the counting frame is not visible in your tracing window, it may be larger than the current field of view.
Re-sizing the counting frame
- Enter new values for Size (side length for the square shape) or for X and Y (for the rectangle shape).
OR
- Drag the sides of the frame with the mouse. The dimensions are updated in the Define Counting Frame window.
Changing the shape from a square (default) to a rectangle
- Place the mouse over the frame, right-click and select Force Square.
OR
- Go to the Options>Stereology Preferences>Counting Frame tab and uncheck Force Counting Frame to be Square.
Changing the colors of the counting frame
- Place the mouse over the frame, right-click and select Change Left(Right) Colors.
OR
- Use the Options>Stereology Preferences>Counting Frame tab.
In monochrome mode, the exclusion and inclusion lines of the counting frame are represented by white solid and dashed lines. There is no option to change the color. 
Moving the counting frame
Place the mouse over the frame. When the cursor changes to a four-headed arrow, drag the mouse to move the counting frame to a new location within the tracing window.
Right-click menu
Change Left Colors -- Red by default, indicating the exclusion boundary. You can change this color if it is too difficult to see clearly.
Change Right Colors -- Green by default, indicating the inclusion boundary. You can change this color if it is too difficult to see clearly.
Force Square -- The counting frame will be constrained to be square, i.e. the horizontal and vertical dimensions will be equal, if this option is chosen.
Accept Count Frame -- Accepts the current size and location of the counting frame.
Cancel -- The definition of a new counting frame can be canceled using this option.
Stereo Investigator 11 | MBF Bioscience Support Center | Downloads